Preliminary Metabolomics Analysis of Urine Serum Samples from Pediatric Dengue Patients Age One Month to 14 Years Old Living at Southern Philippines
Conference Poster Presentation, Coron Westown Resort, Coron, Palawan, Philippines
Our poster was a presentation of the preliminary results of the data coming from the “Mother and Child Determinants of Pediatric Dengue and Development of a Risk Assessment Scoring System for Severe Disease among Filipino Children” Project of the Research and Development Center for Maternal and Child Health (ReDMatCH) Program under the Center for Research and Development, Davao Medical School Foundation Inc. The results primarily highlight both the statistically and biologically significant metabolites that explain dengue severity among children one month to 14 years old. We used urine serum samples and analyzed to Waters Xevo G3 Quadrupole Time-of-Flight (QTOF) Mass Spectrometer, then Waters Acquity Ultra Performance Liquid Chromatography (UPLC) I-Class System, then to Progenesis QI software. The resulting data was then subjected to several advanced statistical methods such as Principal Component Analysis, Partial Least Squares-Discriminant Analysis, Fold Change Analysis, ANOVA, and Elastic Net Regression. This is to understand how changes in metabolites influence the severity of dengue, and pinpoint which of these metabolites play a major role in the disease. See more here.